Oligodeoxyribonuclsotide-3′-phosphate Synthesis by Selective Cleavage of 3′-Terminal Uridine
- 1 February 1986
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 5 (1) , 33-43
- https://doi.org/10.1080/07328318608081904
Abstract
A universal method is evolved for synthesizing oligodeoxyribonucleotides containg a 3′-terminal phosphate group. This method consists of obtaining oligodeoxyribonucleotides containing a 3′-terminal uridine (incorporated by enzyme TdT-catalyzed reaction or by routine triester technique), in selective oxidation of the 3′-terminal ribose of uridine and subsequent β-elimination of the phosphate residue.This publication has 10 references indexed in Scilit:
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