ACTINOMYCIN D INHIBITION OF MICROSOMAL-BOUND HYDROXYPROLINE FORMATION IN RABBIT EMBRYO SKIN IN VITRO
- 1 March 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (3) , 613-619
- https://doi.org/10.1073/pnas.53.3.613
Abstract
The biosynthesis of collagen has been studied in vitro using rabbit embryo skin. Time course studies of proline incorporation into the microsomal fraction indicated a significant uptake into protein-bound proline and into collagen hydroxyproline. Maximally, proline was incorporated in the ratio of 101 of that of hydroxyproline. Uridine-2-C14 was used to measure RNA synthesis, and actinomycin D was the inhibitor utilized to block nuclear RNA formation. The data suggest that actinomycin D inhibition of RNA synthesis also blocked the formation of microsomal-bound hydroxyproline. Hence, the inactivity of the system following the treatment with actinomycin D suggests that collagen messenger RNA is extremely unstable.Keywords
This publication has 17 references indexed in Scilit:
- Kinetics of Incorporation of Uridine-C14 into L Cell RNABiophysical Journal, 1964
- THE NUMBER OF SOLUBLE RNA MOLECULES ON RETICULOCYTE POLYRIBOSOMESProceedings of the National Academy of Sciences, 1964
- THE STABILITY OF LIVER MESSENGER RNAProceedings of the National Academy of Sciences, 1964
- Synthesis of Collagen on PolyribosomesNature, 1964
- The molecular basis of histidase induction in Bacillus subtilisJournal of Molecular Biology, 1963
- X-Ray Diffraction and Molecular Model Building Studies of The Interaction of Actinomycin with Nucleic AcidsNature, 1963
- Studies with a cell-free system of proline incorporation into protein cleaved by collagenaseArchives of Biochemistry and Biophysics, 1963
- DEMONSTRATION OF AN UNSTABLE RNA AND OF A PRECURSOR TO RIBOSOMAL RNA IN HELA CELLSProceedings of the National Academy of Sciences, 1963
- ACTION OF ACTINOMYCIN DON ANIMAL CELLS AND VIRUSEProceedings of the National Academy of Sciences, 1962
- METABOLIC STUDIES OF BONE IN VITRO .4. COLLAGEN BIOSYNTHESIS BY SURVIVING BONE FRAGMENTS IN VITRO1962