EFFECT OF CHLORAMPHENICOL ON PROTEIN AND NUCLEIC ACID METABOLISM IN AZOTOBACTER AGILIS

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Abstract
Incorporation of the carbon of C14-glycine into the ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein of A. agilis is uniformly inhibited by both cobalt and uranyl ions. Chloramphenicol at high concentrations inhibits incorporation of glycine carbon into RNA, DNA, and protein, but at lower concentrations incorporation into only DNA and protein is inhibited to any great extent. Glycine carbon is incorporated initially into a fraction that is not sedimentable in 180 minutes at 144,000 x G; it is in this fraction that incorporation into DNA and protein is most strongly inhibited while incorporation into RNA is inhibited very little.