An Integrated Microfabricated Cell Sorter
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- 27 April 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 74 (11) , 2451-2457
- https://doi.org/10.1021/ac0255330
Abstract
We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is ∼100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.Keywords
This publication has 14 references indexed in Scilit:
- From Micro- to Nanofabrication with Soft MaterialsScience, 2000
- A/C WebWorks: What’s in a genome?Analytical Chemistry, 2000
- Monolithic Microfabricated Valves and Pumps by Multilayer Soft LithographyScience, 2000
- Measurement of kinase activation in single mammalian cellsNature Biotechnology, 2000
- Replication and Compression of Surface Structures with Polydimethylsiloxane ElastomerJournal of Chemical Education, 1999
- Monoclonal antibody binding affinity determined by microchip‐based capillary electrophoresisElectrophoresis, 1998
- MastheadAnalytical Chemistry, 1998
- Integrated Capillary Electrophoresis on Flexible Silicone Microdevices: Analysis of DNA Restriction Fragments and Detection of Single DNA Molecules on MicrochipsAnalytical Chemistry, 1997
- Large-area travelling-wave dielectrophoresis particle separatorJournal of Micromechanics and Microengineering, 1997
- Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a ChipScience, 1993