In vivo localization of a bispecific antibody which targets human t lymphocytes to lyse human colon cancer cells

Publisher Website
Google Scholar
Abstract
A bispecific MAb was derived from the fusion of a hybridoma producing MAb CD3 with a hybridoma producing MAb L‐Dl (which is directed against a 41‐kDa glycoprotein expressed in most gastro‐intestinal and pancreatic carcinomas). Bispecific antibody molecules were isolated from parental antibody molecules by the use of hydroxylapatite‐HPLC and shown to target human cytolytic T lymphocytes, irrespective of their original specificity, to specifically lyse human colon carcinoma cells. Localization studies in vivo using nude mice bearing human colon carcinoma xenografts showed significant accumulation of the HPLC‐purified 125l‐labelled bispecific antibodies into the tumor compared to 131l‐labelled control CD3 antibody.
Keywords