Cloning and Sequencing of themeso-Diaminopimelate-d-dehydrogenase (ddh) Gene ofCorynebacterium glutamicum
- 1 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 52 (11) , 2903-2909
- https://doi.org/10.1080/00021369.1988.10869162
Abstract
The ddh gene of Corynebacterium glutamicum encoding raesodiaminopimelate meso-diaminopimelate (meso-DAP)-d-dehydrogenase (DDH) involved in the lysine biosynthesis was cloned in a DAP auxotroph (dapD4) of Escherichia coli by complementation of the DAP auxotroph. Deletion analysis revealed that a ~1.7-kb XhoI-KpnI fragment contained the ddh structure gene. The specific activity of DDH was increased fourteen-fold when C. glutamicum was transformed with a recombinant plasmid harbouring the cloned ddh gene. Furthermore, the ddh gene has been sequenced [S. Ishino et al., Nucleic Acids Res., 15, 3917 (1987)] and some properties of the ddh gene are discussed.This publication has 13 references indexed in Scilit:
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