Structure-independent nucleotide sequence analysis.
- 1 May 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (5) , 2232-2235
- https://doi.org/10.1073/pnas.76.5.2232
Abstract
Substitution of inosine for guanosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its MW. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.This publication has 33 references indexed in Scilit:
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