Reinvestigation of the primary structure of yeast alanine tRNA

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Abstract
The primary structure of alanine transfer RNA reported by Holley and his colleagues contained a number of inconsistencies between the data and the proposed sequence. In order to resolve these inconsistencies, alanine tRNA was isolated from yeast by using countercurrent techniques similar to those employed by Holley. The alanine accepting RNA obtained by this procedure was found by further purification employing reverse phase partition chromatography, to contain two different species of alanine tRNA. One of these, tRNAala I, could be shown to be similar to Holley's proposed structure with the addition of a guanine between positions 47 and 48 and only 2 dihydrouridines instead of the 2.5 reported by Holley.