Transmission electron microscopy of small numbers of sorted cells
- 1 January 1988
- Vol. 9 (1) , 88-92
- https://doi.org/10.1002/cyto.990090114
Abstract
A new method that allows the transmission electron microscopic examination of as few as 1 × 104 cells obtained by flow cytometric sorting is described. The approach involves “sandwiching” fixed cells in an agarose case by a microcentrifugation system consisting of small‐diameter cell‐centrifugation tubes and subsequent processing of the cells by conventional techniques. The advantages offered by this method are discussed.Keywords
This publication has 17 references indexed in Scilit:
- Identification and isolation of subpopulations of pleural cells by multiparameter flow cytometryCell Biochemistry and Biophysics, 1986
- Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cellsJournal of Immunological Methods, 1984
- A polycarbonate filter technique for collection of sorted cellsCytometry, 1982
- A Rapid Technique for Preparing Microorganisms for Transmission Electron MicroscopyStain Technology, 1979
- A Beem Capsule Chamber-Pipette for Handling Small Specimens for Electron MicroscopyStain Technology, 1974
- A filtration technique for preparing cells in suspension for electron microscopyJournal of Microscopy, 1970
- Embedding Free-Floating Cells and Microscopic Particles: Serum Albumin Coagulum—Epoxy ResinStain Technology, 1968
- USE OF A MICROCENTRIFUGE FOR PREPARATION OF ISOLATED MITOCHONDRIA AND CELL SUSPENSIONS FOR ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961
- NEW EMBEDDING METHOD FOR CELL SUSPENSIONSThe Journal of cell biology, 1961