TNF and IL‐6 are potent growth factors for OH‐2, a novel human myeloma cell line

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Abstract
A novel human myeloma cell line, OH‐2, was established from pleural fluid of a myeloma patient in end stage of the disease. Effects of cytokines on proliferation were analyzed by measuring uptake of 3H‐thymidine. Cell surface antigens were detected by flow cytometry. The cell line is dependent on IL‐6 for growth and proliferates in response to TNF. There is synergy between the stimulatory response of TNF and IL‐6. The cells express both the p55 and p75 TNF receptors. Neutralizing anti‐IL‐6 did not inhibit TNF‐mediated proliferation, showing that TNF acts through a pathway that is independent of IL‐6. TNF was more potent than IL‐6 in stimulating the growth of primary myeloma cultures (>99% pure) from the same patient (OH‐2‐PC), indicating that TNF in selected myeloma patients has a growth‐promoting effect equal to IL‐6. OH‐2 cells produce and secrete monoclonal IgG‐kappa.