Regulation of the threonine operon: tandem threonine and isoleucine codons in the control region and translational control of transcription termination.
- 1 April 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (4) , 1706-1710
- https://doi.org/10.1073/pnas.76.4.1706
Abstract
The DNA sequence of 178 base pairs preceding the 1st structural gene of the threonine operon of Escherichia coli was determined. A region of perfect 2-fold rotational symmetry, involving 28 base pairs, precedes the 1st structural gene. The structural similarity of this sequence to known RNA polymerase termination sites suggests that this region is the termination site of the threonine operon leader RNA. A mutation (thr 79-20), which confers a derepressed, constitutive phenotype, was sequenced and it was a G .cntdot. C insertion in the putative terminator. A potential coding region for a 21-amino acid leader peptide ends about 18 base pairs before the terminator. This peptide contains 8 threonine and 4 isoleucine codons. Eleven of these codons are in tandem. A model for threonine operon regulation, involving alternative secondary RNA structures and translation of leader RNA, is discussed.This publication has 23 references indexed in Scilit:
- Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon.Proceedings of the National Academy of Sciences, 1978
- Identification and restriction endonuclease mapping of the threonine operon regulatory regionJournal of Molecular Biology, 1978
- Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.Proceedings of the National Academy of Sciences, 1978
- DNA sequence from the histidine operon control region: seven histidine codons in a row.Proceedings of the National Academy of Sciences, 1978
- Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1978
- Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coliProceedings of the National Academy of Sciences, 1978
- New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12Molecular Genetics and Genomics, 1978
- Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.Proceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Multivalent repression in the biosynthesis of threonine in Salmonella, typhimurium and Escherichia, coliBiochemical and Biophysical Research Communications, 1963