Simplified method for typing herpes simplex virus by restriction endonuclease analysis
- 1 March 1983
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 17 (3) , 548-51
- https://doi.org/10.1128/jcm.17.3.548-551.1983
Abstract
We have developed a simplified method for unambiguously typing herpes simplex virus. The method depends on the production of cell-associated virus at 34 degrees C and subsequently, on the separation of cellular DNA and viral DNA by Dounce homogenization and the removal of nuclei by centrifugation. Viral nucleic acid was prepared from the cytoplasmic fraction and analyzed after restriction endonuclease cleavage. The method obviates the use of radioactive isotopes, and the viral DNA is effectively free of interfering cellular DNA.Keywords
This publication has 13 references indexed in Scilit:
- A simple method for typing clinical isolates of herpesvirus simplex: replication in the presence of 2h-1,3-oxazine-2,6 (3h)-dione (oxauracil)Journal of Medical Virology, 1980
- A RAPID TECHNIQUE FOR DISTINGUISHING HERPES-SIMPLEX VIRUS TYPE 1 FROM TYPE 2 BY RESTRICTION-ENZYME TECHNOLOGYThe Lancet, 1979
- Restriction Endonuclease Fingerprinting of Herpes Simplex Virus DNA: A Novel Epidemiological Tool Applied to a Nosocomial OutbreakThe Journal of Infectious Diseases, 1978
- The Use of Temperature Sensitivity and Selective Cell Culture Systems for Differentiation of Herpes Simplex Virus Types 1 and 2 in a Clinical LaboratoryExperimental Biology and Medicine, 1977
- A new method for the isolation of herpes simplex virus type 2 DNAVirology, 1976
- Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.1974
- Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique.1971
- Differentiation between Type 1 and Type 2 Strains of Herpes Simplex Virus by an Indirect Immunofluorescent TechniqueJournal of General Virology, 1971
- Typing of Herpesvirus hominis Strains by a Direct Immunofluorescent TechniqueExperimental Biology and Medicine, 1969
- The effect of the temperature of incubation on the formation and release of herpes simplex virus in infected FL cellsVirology, 1959