N‐Acetylglucosaminyltransferase III, IV and V activities in Novikoff ascites tumour cells, mouse lymphoma cells and hen oviduct
- 1 May 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 181 (3) , 651-655
- https://doi.org/10.1111/j.1432-1033.1989.tb14772.x
Abstract
A specific and fast method for the determination of N-acetylglucosaminyltransferase III, IV and V activity in one assay is described. The method is based on the separation by HPLC of the three transferase products formed from the common acceptor oligosaccharide substrate GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 3(GlcNAc.beta.1 .fwdarw. 2 Man.alpha.1 .fwdarw. 6) Man .beta.1 .fwdarw. 4GlcNAc. Assays are not interfered with by substances that result from enzymatic or chemical breakdown of the donor substrate UDP-[14C]GlcNAc. Using this assay system N-acetylglucosaminyltransferase III, IV and V activities were estimated in Novikoff ascites tumour cells, mouse lymphoma BW 5147 cells and hen oviduct.This publication has 31 references indexed in Scilit:
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