High‐throughput liquid chromatography ultraviolet/mass spectrometric analysis of combinatorial libraries using an eight‐channel multiplexed electrospray time‐of‐flight mass spectrometer
- 28 June 2002
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (15) , 1440-1447
- https://doi.org/10.1002/rcm.731
Abstract
We report a high-throughput liquid chromatography/mass spectrometry (LC/MS) protocol for analyzing large combinatorial libraries using an eight-channel parallel LC/UV/MS (MUX-LCT) system. System configuration, linear response range in UV absorbance, LC column selection, and flow rate were optimized for 24 h/7 day unattended operations. Combinatorial libraries were analyzed on this system at a rate of 3200 compounds per day for a 3.5 min cycle time per injection. This parallel system is compared with a single-channel system in terms of performance and operation. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
This publication has 11 references indexed in Scilit:
- An Integrated Ten-Pump, Eight-Channel Parallel LC/MS System for Automated High-Throughput Analysis of ProteinsAnalytical Chemistry, 2001
- Fast characterization of intact proteins using a high‐throughput eight‐channel parallel liquid chromatography/mass spectrometry systemRapid Communications in Mass Spectrometry, 2001
- Evaluation of a Four-Channel Multiplexed Electrospray Triple Quadrupole Mass Spectrometer for the Simultaneous Validation of LC/MS/MS Methods in Four Different Preclinical MatrixesAnalytical Chemistry, 2001
- Expediting the Method Development and Quality Control of Reversed-Phase Liquid Chromatography Electrospray Ionization Mass Spectrometry for Pharmaceutical Analysis by Using an LC/MS Performance Test MixAnalytical Chemistry, 2000
- Parallel ultra-high flow rate liquid chromatography with mass spectrometric detection using a multiplex electrospray source for direct, sensitive determination of pharmaceuticals in plasma at extremely high throughputRapid Communications in Mass Spectrometry, 2000
- Analytical Methods in Combinatorial ChemistryCritical Reviews in Combinatorial Chemistry, 2000
- FTICR-mass spectrometry for high-resolution analysis in combinatorial chemistryBiotechnology & Bioengineering, 2000
- High-throughput determination of identity, purity, and quantity of combinatorial library members using LC/MS/UV/ELSDBiotechnology & Bioengineering, 2000
- High throughput liquid chromatography/mass spectrometric analyses using a novel multiplexed electrospray interfaceRapid Communications in Mass Spectrometry, 1999
- Packed Column Supercritical Fluid Chromatography/Mass Spectrometry for High-Throughput AnalysisAnalytical Chemistry, 1999