A Detailed Serological Study of Five Anti‐Jka Sera Reacting by the Antiglobulin Technique

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Abstract
Detailed serological investigation of five examples of anti‐Jka revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of complement, while two were detectable with anti‐IgG, providing a spin test was used. Complement was bound by all and resulted in enhanced reactions, particularly when slide/tile tests were used. The study emphasizes that anti‐complement (C3) is an essential component of polyspecific antiglobulin reagents used in cross‐matching, enabling the ready detection of these clinically important antibodies.