siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference
Open Access
- 1 July 2004
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (Web Server) , W124-W129
- https://doi.org/10.1093/nar/gkh442
Abstract
SiDirect (http://design.RNAi.jp/) is a web-based online software system for computing highly effective small interfering RNA (siRNA) sequences with maximum target-specificity for mammalian RNA interference (RNAi). Highly effective siRNA sequences are selected using novel guidelines that were established through an extensive study of the relationship between siRNA sequences and RNAi activity. Our efficient software avoids off-target gene silencing to enumerate potential cross-hybridization candidates that the widely used BLAST search may overlook. The website accepts an arbitrary sequence as input and quickly returns siRNA candidates, providing a wide scope of applications in mammalian RNAi, including systematic functional genomics and therapeutic gene silencing.Keywords
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