Protein recovery from blood plasma by precipitation with polyuronates

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Abstract
Summary: Bovine serum albumin (BSA) and blood plasma formed insoluble complexes with alginate, pectate and carboxymethyl cellulose (CMC) under appropriate conditions of pH. More than 90% of the BSA could be recovered by all three polysaccharides at ionic strengths of < 0.001 and at the optimum pH for recovery and a ratio of protein to polysaccharide of about 5:1. At low ratios of protein to polysaccharide, alginate and CMC appeared to resolubilize the complex. When the ionic strength was increased to 0.2 almost complete recovery could still be obtained with alginate and pectate at protein: polysaccharide ratios of 5:1 and 3:1 respectively but the efficiency of precipitation was reduced and the range of the ratios for optimum recovery narrowed. The results for precipitation from blood plasma were very similar to those obtained in the BSA studies. A crude pectate preparation obtained from orange peel gave results similar to pure pectate except that three times as much was required to precipitate a given amount of protein.