Mobilization of plasmid pHSV106 from Escherichia coli HB101 in a laboratory-scale waste treatment facility
- 1 April 1987
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 53 (4) , 665-671
- https://doi.org/10.1128/aem.53.4.665-671.1987
Abstract
The mobilization of plasmid pHSV106 from Escherichia coli HB101, in a laboratory model waste treatment facility, by both laboratory and indigenous wastewater strains of E. coli was monitored by transfer of antibiotic resistance characteristics and by detection of pHSV106 DNA sequences in recipient cells. The mobilization was demonstrated to occur under several different treatment conditions, such as different media composition, microbial concentrations, and waste flow rates. The herpes simplex virus thymidine kinase gene was used as a hybridization marker to confirm the occurrence of the transfer. The use of the HB101 (recA mutant) host cell implies that recA functions are unnecessary for the gene transfer.This publication has 12 references indexed in Scilit:
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