Isolation of frameshift mutagens from smokers' urine: experiences with three concentration methods
- 30 April 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (5) , 783-788
- https://doi.org/10.1093/carcin/6.5.783
Abstract
Frameshift mutagens were isolated and concentrated from cigarette smokers' urine by the use of three different extraction methods; XAD-2 resin chromatography, chloroform and blue cotton extraction systems. The extracts were tested for mutagenicity in the Salmonella/mammalian microsome test employing Salmonella typhimurium TA98. By the use of XAD-2 resin chromatography, no clear correlation was found between urine mutagenicity versus number of cigarettes smoked and no detectable mutagenic activity was observed when fewer than four cigarettes a day were con sumed. A clear correlation was found between urine muta genicity versus number of cigarettes smoked when urine samples were extracted by the use of chloroform or blue cotton. Interference of free histidine in mutagenicity assays could be excluded.This publication has 19 references indexed in Scilit:
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