The identity of conformational states of reconstituted and native histone octamers
- 1 December 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 137 (1-2) , 221-226
- https://doi.org/10.1111/j.1432-1033.1983.tb07818.x
Abstract
Histone octamers were reconstituted from the following preparations: natural histone H3-H4 tetramer and histone H2A-H2B dimer, either selectively extracted from chromatin with solutions of NaCl or prepared by dissociation of the natural octamer; acid-denaturated core histones, either an unfractionated mixture or individually purified proteins. Complexes assembled from these histones elute from exclusion chromatography columns with octamer size as verified by cross-linking with dimethylsuberimidate. The reconstituted octamers all crystallize in the same form of helical tubes as the natural octamer.This publication has 20 references indexed in Scilit:
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