Detection of the c‐myc Gene Product in Urinary Bladder Cancer
Open Access
- 1 December 1990
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 81 (12) , 1198-1201
- https://doi.org/10.1111/j.1349-7006.1990.tb02677.x
Abstract
Expression of the c‐myc gene product in urinary bladder cancer was investigated by immunohistochemical staining with anti‐c‐myc monoclonal antibody (mAb) MYC‐1. Positive staining was observed in the cytoplasm, but not in the nucleus in tissues fixed with 10% formalin. On the other hand, positive staining was localized in the nucleus in cryopreserved tissues. Of 34 cryopreserved specimens examined, positive staining with MYC‐1 mAb was observed in 1 of 12 (8.3%) of grade 1 (G1), 12 of 15 (80%) of G2 and 6 of 7 (86%) of G3. Positive staining with Ki‐67 mAb was observed in 2 of 12 (17%) of G1, 12 of 15 (80%) of G2, and 6 of 7 (86%) of G3. These results suggest that tumors with higher nuclear pleomorphism contain more proliferating cells.Keywords
This publication has 16 references indexed in Scilit:
- Diversity of Cellular Molecules in Human Cells Detected by Monoclonal Antibodies Reactive with c-mycProteins Produced inEscherichia coliJapanese Journal of Cancer Research, 1989
- PREPARATION OF ANTI-RAS MR 21,000 PROTEIN MONOCLONAL-ANTIBODIES AND IMMUNOHISTOCHEMICAL ANALYSES ON EXPRESSION OF RAS GENES IN HUMAN STOMACH AND THYROID CANCERS1988
- A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1Nature, 1987
- c-myc oncogene expression in colorectal cancerCancer, 1987
- Participation of c- myc Protein in DNA Synthesis of Human CellsScience, 1986
- Cell surface antigens of human bladder cancer defined by mouse monoclonal antibodies.Proceedings of the National Academy of Sciences, 1984
- Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factorCell, 1983
- Identification of nuclear proteins encoded by viral and cellular myc oncogenesNature, 1983
- Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferationInternational Journal of Cancer, 1983
- Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virusNature, 1982