Fluorescent Pseudomonas species categorized by using polymerase chain reaction (PCR)/restriction fragment analysis of 16S rDNA
- 1 October 1994
- journal article
- Published by Wiley in Molecular Ecology
- Vol. 3 (5) , 479-487
- https://doi.org/10.1111/j.1365-294x.1994.tb00126.x
Abstract
A rapid procedure for the identification of fluorescent pseudomonads, based on the polymerase chain reaction (PCR) and restriction fragment analysis of 16S rDNA genes is described. Thirty-one strains belonging to 10 different Pseudomonas species of the Pseudomonas fluorescens rRNA branch were characterized. Amplified rDNA was digested with 13 different restriction endonucleases. The combined data from restriction analysis enabled the definition of 17 different 16S rDNA genotypes. All type strains belonging to different species were differentiated. The good correlation between grouping obtained using restriction analysis with other molecular classification criteria demonstrates the value of the described method to characterize rapidly fluorescent Pseudomonas strains at the species level.Keywords
This publication has 50 references indexed in Scilit:
- The interrelationships of all major groups of Platyhelminthes: phylogenetic evidence from morphology and moleculesBiological Journal of the Linnean Society, 1999
- Suppression of fusarium wilts by fluorescent pseudomonads: Mechanisms and applicationsBiocontrol Science and Technology, 1993
- DNA Relatedness among the Pathovar Strains of Pseudomonas syringae subsp. savastanoi Janse (1982) and Proposal of Pseudomonas savastanoi sp. nov.International Journal of Systematic and Evolutionary Microbiology, 1992
- Classification of medically important clostridia using restriction endonuclease site differences of PCR-amplified 16S rDNAJournal of General Microbiology, 1991
- Deoxyribonucleic Acid Similarities among Pseudomonas SpeciesInternational Journal of Systematic and Evolutionary Microbiology, 1989
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences, 1979
- The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical PropertiesJournal of General Microbiology, 1978
- Deoxyribonucleic Acid Homology Groups Among Phytopathogenic Pseudomonas SpeciesInternational Journal of Systematic and Evolutionary Microbiology, 1973
- The Aerobic Pseudomonads a Taxonomic StudyJournal of General Microbiology, 1966