Effect of Insulin on Glucose Uptake by Normal and Neoplastic Mouse Mammary Tissues in Organ Culture2

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Abstract
The glucose uptake of expiants of normal prelactating mammary lobules and of spontaneous mammary adenocarcinomas of C3H/Crgl mice was compared in organ culture in the presence and absence of insulin. In the absence of insulin the normal explants used little or no glucose after the 1st day, whereas tumor explants used glucose at a high rate throughout the 5-day culture period. The presence of insulin resulted in an increase in the daily glucose uptake by normal explants but had no effect on tumor explants. As the concentration of insulin was increased from 0 to 40 µg per ml, normal explants showed a corresponding increased rate of glucose uptake; at concentrations above 40 µg per ml, the rate decreased. Tumor explants showed no response to insulin at any concentration.