The Release of Ammonia Nitrogen from Urea, Ammoniated Molasses, and Soybean Oil Meal in the Presence of Rumen Microorganisms,

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Abstract
Four series of experiments were employed to determine the relative amounts of free ammonia nitrogen associated with urea, ammoniated molasses, 15.4% protein equivalent, ammoniated invert molasses, 33.3% protein equivalent and soybean oil meal in the presence of rumen microflora and the enzyme urease. Evidence is presented to indicate that there was some free ammonia in both samples of ammoniated molasses. The amount of free ammonia associated with these materials did not increase significantly with prolonged fermentation in an artificial rumen nor was there a significant amount released in the presence of the enzyme urease. Considerable amounts of ammonia nitrogen are released from urea in the presence of microflora both in vitro and in vivo and in the presence of the enzyme urease. Small amounts of ammonia nitrogen are released from soybean oil meal in the presence of rumen microflora and the enzyme urease.