Nutritional Studies on Desulfovibrio desulfuricans using Chemically Defined Media

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Abstract
A non-precipitating chemically defined medium containing lactate, sulphate and other inorganic salts supported repeated subculture of D. desulturicans, strain Hildenborough; yields of bacteria were comparable with those obtained in media containing yeast extract or peptone. Addition of yeast extract, amino-acid mixtures of ATP to the defined medium increased the crop. Growth on other organic substances was poorer than on lactate; amino-acids were less efficient nitrogen sources than ammonia. Pyruvate served as electron acceptor for hydrogen uptake by resting organisms but did not support growth in sulfate-free medium.