The Products of Gene US11 of Herpes Simplex Virus Type 1 Are DNA-binding and Localize to the Nucleoli of Infected Cells
- 1 July 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (7) , 1921-1937
- https://doi.org/10.1099/0022-1317-68-7-1921
Abstract
We have used antisera raised against synthetic oligopeptides to characterize the protein products from herpes simplex virus type 1 gene Us11. These antisera recognized predominantly polypeptides of apparent molecular weight 21,000 and 22,000, but also polypeptides of apparent molecular weight 17,500, 15,000, 14,000 and 11,000. Tryptic peptide fingerprint analysis confirmed that these polypeptides were all closely related. The 21,000 and 22,000 molecular weight polypeptides were shown to be DNA-binding proteins, and immune electron microscopy demonstrated their strong localization within nucleoli of infected cells.This publication has 23 references indexed in Scilit:
- BamI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNAJournal of Virology, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The structure and isomerization of herpes simplex virus genomesCell, 1979
- A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extractionAnalytical Biochemistry, 1979
- Anatomy of Herpes Simplex Virus DNA XII. Accumulation of Head-to-Tail Concatemers in Nuclei of Infected Cells and Their Role in the Generation of the Four Isomeric Arrangements of Viral DNAJournal of Virology, 1979
- Physical mapping of herpes simplex virus-induced polypeptidesJournal of Virology, 1978
- Quantitative correlation of morphological alterations of the nucleus with functional events during in vitro infection of glial cells with herpes simplex hominis (HSV 2)Journal of Ultrastructure Research, 1978
- Structure of the joint region and the termini of the DNA of herpes simplex virus type 1Journal of Virology, 1978
- Anatomy of Herpes Simplex Virus DNA. VIII. Properties of the Replicating DNAJournal of Virology, 1977
- DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cellsJournal of Virology, 1976