THE MICROCOLORIMETRIC DETERMINATION OF INORGANIC PHOSPHATE IN PLASMA AND URINE: I. QUANTITATIVE INVESTIGATION OF PRESENT COMMON PROCEDURES II. A SENSITIVE AND STABLE METHOD FOR PLASMA, SERUM, AND URINE
- 1 October 1949
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Research
- Vol. 27e (5) , 265-274
- https://doi.org/10.1139/cjr49e-036
Abstract
A quantitative investigation of the common colorimetric procedures used for determining inorganic, phosphate was made to determine the factors affecting the sensitivity and color stability. A method is presented that is accurate to within ± 2% and is sufficiently sensitive to give quantitative color development with less than 1 γ of phosphorus. The color produced is quite stable for colorimetry. The method has been adapted to the determination of phosphorus in 0.1 or 0.02 ml. of plasma, serum, or urine or in the protein-free filtrate residue from the determination of sodium, potassium, calcium, and chloride in 0.4 ml. of plasma.Keywords
This publication has 3 references indexed in Scilit:
- THE DETERMINATION OF SERUM PHOSPHATE BY THE MOLYBDIVANADATE METHODJournal of Biological Chemistry, 1946
- Colorimetric Determination of Phosphorus as Molybdivanadophosphoric AcidIndustrial & Engineering Chemistry Analytical Edition, 1944
- An improved method for the colorimetric determination of phosphateBiochemical Journal, 1938