Interrelationships of Catalase, Peroxidase, Hematin, and Chlorophyll

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Abstract
A study was made of the catalase and peroxidase activities as well as of chlorophyll and total hematin contents of mustard plants. The plants were grown in nutrient culture with different Fe, K, C, P and N levels. Leaves of Ni-toxic and variegated plants were studied also. Peroxidase activity showed only small variations between chlorotic and green leaves, whereas catalase activity was several fold greater in green than in chlorotic leaves. A linear relationship of 60:1 between chlorophyll and hematin was found. A spectrophotometric method is reported for determining total hematin compounds as pyridine hemochrome in acetone residues derived from leaves.