The oxidation of manganese by peroxidase systems

Abstract
Plant peroxidase prepns. from horseradish and turnip in the presence of certain phenolic substrates and H2O2 were shown to oxidize manganous salts. Oxidation of Mn was demonstrated with phenol, p-cresol, o-cresol and resorcinol as the phenolic substrate, but not with quinol, catechol, pyrogallol and caffeic acid. The effect of variation in the concns. of peroxidase, phenolic substrate, and H2O2 was studied. An active system was demonstrated with 1 [mu]g. of p-cresol. The results were in agreement with the hypothesis previously advanced that the Mn reduced an oxidation product of the phenolic substrate and thereby was itself oxidized.

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