EFFECT OF ALTERATIONS IN THE QUALITY AND QUANTITY OF DIETARY-FAT ON 1,2-DIMETHYLHYDRAZINE-INDUCED COLON TUMORIGENESIS IN RATS

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (9) , 4083-4090
Abstract
The effect of alterations in the quality and quantity of dietary fat on 1,2-dimethylhydrazine-induced colon cancer in rats was studied. Weanling Sprague-Dawley rats were fed semipurified diets containing 24% beef fat, 24% corn oil, 24% Crisco or the 3 fats in equal parts to make a total of 5% fat with other macronutrients and micronutrients adjusted to balance the ratios of nutrient to calorie. After 4 wk of dietary treatment, all rats, except vehicle-treated animals, received 1,2-dimethylhydrazine (15 mg/kg) by gavage, once/wk for 5 wk. The animals were fed the experimental diets until intestinal tumors developed, and surviving animals were sacrificed at 60 wk. There was no effect of any high-fat diet on intestinal tumor incidence, latency, size or frequency. All groups contained the same proportion of adenomas (< 3%) and adenocarcinomas classified as mucinous. In the group fed 24% Crisco, tumors occurred with greater frequency in the proximal section of the colon than in lower segments, but the distribution was approximately uniform in the other groups. Cumulative probability of death with colon carcinoma was lowest in the 24% Crisco group, but the other high-fat did not differ significantly from the 5% mixed fat group or from one another.

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