Random cloning of bent DNA segments fromEscherichia colichromosome and primary characterization of their structures
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (17) , 6827-6841
- https://doi.org/10.1093/nar/15.17.6827
Abstract
A simple method for the selective detection of DNA segments containing a sequence-directed static bend was developed. Two-dimensional polyacrylamide gel electrophoresis performed at two different temperatures (60°C and 10°C) can effectively separate a bent DNA from a mixture of normal DNA. Using this method, a bank of plasmids carrying bent DNA inserts from the E. coli total chromosome was constructed. The primary characterization of a set of bent DNA segments randomly cloned from E. coli was presented.Keywords
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