Formation of haematins and haemochromogens

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Abstract
Comparison of colors given by solns. of several hematins and hemochromogens prepared from Hb and from hemin favors the theory that a conjugated protein is formed when blood is dissolved in O.1N-NaOH, pyridine, or diethylamine, with hematin in the oxidized state. The hematins formed by Hb when treated with diethylamine and with pyridine, and the hemochromogen it forms with pyridine, appear to be different from those formed by hemin, and may be mixed compounds containing globin as well as the organic base. The pyridine hemochromogen from purified Hb differs slightly in the position of its spectral bands from that formed from blood.

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