Mutational Activation of H‐ras and K‐ras Genes Is Absent in N‐Nitroso‐N‐methylurea‐induced Liver Tumors in Rats
Open Access
- 1 May 1990
- journal article
- research article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 81 (5) , 437-439
- https://doi.org/10.1111/j.1349-7006.1990.tb02587.x
Abstract
We examined mutational activation of H‐ and K‐ras genes in hyperplastic nodules and hepatocellular carcinomas induced by N‐nitroso‐N‐methylurea or diethylnitrosamine using the polymerase chain reaction, followed by dot‐blot hybridization. No mutational activation was detected in these hepatic lesions. The results indicate that low incidence of the activation of H‐ and K‐ras genes in rat liver tumors is due to the organ specificity rather than the nature of the carcinogens used.Keywords
This publication has 24 references indexed in Scilit:
- Low Incidence of Point Mutation of c-Ki-rasand N-rasOncogenes in Human Hepatocellular CarcinomaJapanese Journal of Cancer Research, 1989
- Mutations at codon 61 of the Ha‐ras proto‐oncogene in precancerous liver lesions of the B6C3F1 mouseMolecular Carcinogenesis, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Detection of activated proto-oncogenes in N-nitrosodiethylamine-induced liver tumors: a comparison between B6C3F1 mice and Fischer 344 ratsCarcinogenesis: Integrative Cancer Research, 1988
- Activated Oncogenes in B6C3F1 Mouse Liver Tumors: Implications for Risk AssessmentScience, 1987
- ras GENESAnnual Review of Biochemistry, 1987
- A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotidesGene, 1986
- Direct mutagenesis of Ha-ras-1 oncogenes by N-nitroso-N-methylurea during initiation of mammary carcinogenesis in ratsNature, 1985
- Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutationsNature, 1983
- Initiation of chemical carcinogenesis requires cell proliferationNature, 1978