MEASUREMENTS OF INVIVO P-31 NUCLEAR MAGNETIC-RESONANCE SPECTRA IN NEUROECTODERMAL TUMORS FOR THE EVALUATION OF THE EFFECTS OF CHEMOTHERAPY

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (6) , 2429-2433
Abstract
The effects of chemotherapy on living tumor tissue in hamsters and rats were investigated by measuring the 31P-NMR spectra using topical magnetic resonance. Human neuroblastoma, human glioblastoma and rat glioma tumor cells were inoculated s.c. in the lumbar region of the animals. After the diameter of the tumors increased to 1.5 cm, in vivo 31P-NMR spectra were measured selectively in the tumors with a TMR-32 spectrometer. ATP Pi, phosphodiester and phosphomonoester peaks were observed. The phosphocreatinine peak was hardly detectable, ATP and phosphomonoester peaks were high and tissue pH, calculated from the chemical shift of Pi, declined. Regardless of the tumor origin or the histological typical the spectra pattern of each neuroectodermal tumor was found to be essentially the same. After i.v. injection of a large dose of a chemotherapeutic agent, ATP peaks decreased and Pi increased gradually, resulting in a dominant Pi peak pattern after 6-12 h. During the same periods, there were no observable changes in the spectra of normal organs. The drugs evidently have a selective and direct action on the energy metabolism of tumor cells. With lower drugs dose, no remarkable changes were seen in the spectrum. Measurement of in vivo 31P-NMR spectra is valuable not only to investigate the energy metabolism in tumor tissue but also to evaluate the effects of chemotherapy.

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