Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide
- 1 April 1991
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 35 (4) , 331-333
- https://doi.org/10.1111/j.1348-0421.1991.tb01562.x
Abstract
Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.Keywords
This publication has 4 references indexed in Scilit:
- Rapid and sensitive silver-lipopolysaccharide staining using PhastSystem in fast horizontal polyacrylamide gel electrophoresisElectrophoresis, 1989
- A Yersinia enterocolitica serotype 0:3 lipopolysaccharide-specific monoclonal antibody reacts more strongly with bacteria cultured at room temperature than those cultured at 37 degrees C.The Journal of Immunology, 1985
- Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gelsJournal of Bacteriology, 1983
- A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gelsAnalytical Biochemistry, 1982