The nucleotide sequence of theBgene of bacteriophage Mu
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (22) , 8627-8638
- https://doi.org/10.1093/nar/12.22.8627
Abstract
Bacteriophage Mu is a highly efficient transposon which requires the products of the Mu A and B genes in order to transpose at a normal frequency. We have determined the nucleotide sequence of the B gene as well as that of the A-B intergenic region upstream of B. The protein product of the gene contains 312 amino acids and has a predicted molecular weight of 35,061. As expected, there do not appear to be any potential promoter sequences in the intergenic region prior to the gene, but it is preceded by a strong Shine-Dalgarno sequence. The intergenic region does not contain any obvious transcription termination sequences. The frequency of optimal codon usage is similar to that for other transposon and phage genes, and the amino acid composition is comparable to that of an “average” E.coli protein. A region near the amino terminus of the protein resembles the highly conserved bihelical fold which is involved in DNA contact and sequence specific recognition in a number of DNA binding proteins.Keywords
This publication has 24 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsPublished by Elsevier ,2006
- Control of phage λ development by stability and synthesis of cll protein: Role of the viral clll and host hflA, himA and himD genesCell, 1982
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Homology among DNA-binding proteins suggests use of a conserved super-secondary structureNature, 1982
- Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational systemJournal of Molecular Biology, 1981
- Predominant end-products of prophage Mu DNA transposition during the lytic cycle are replicon fusionsJournal of Molecular Biology, 1981
- Purified λ regulatory protein cII positively activates promoters for lysogenic developmentNature, 1981
- Polypeptides encoded by the early region of bacteriophage Mu synthesized in minicells of Escherichia coliJournal of Molecular Biology, 1981
- Am E. coli gene product required for λ site-specific recombinationCell, 1980
- Involvement of phage Mu-1 early functions in Mu-mediated chromosomal rearrangementsNature, 1978