BASIS FOR FLUOROPYRIMIDINE-INDUCED ANTAGONISM TO METHOTREXATE IN EHRLICH ASCITES TUMOR-CELLS INVITRO

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (1) , 219-222
Abstract
The inhibitory effect of methotrexate on [3H]deoxyuridine incorporation into DNA is reduced as the basal rate of this reaction is inhibited by pretreatment of Ehrlich ascites mouse tumor cells with fluoropyrimidines. This observation is a basis for fluoropyrimidine-methotrexate antagonism in anticancer regimens and supports the concept that the sensitivity of thymidylate synthesis in tumor cells to methotrexate is related to the basal rate of thymidylate synthesis from deoxyuridylate.