Cultivation of Type 2 Dengue Virus in Rhesus Kidney Tissue Culture.

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Abstract
Sumary and conclusions Mouse-passaged type 2 dengue virus of the New Guinea C strain was cultivated in trypsinized rhesus kidney tissue cultures grown directly on glass surfaces and incubated in the stationary state at 35°C virus was maintained in this system of tissue culture through 18 passages during 176 days. Culture fluid from the 18th passage representing a 10-41.5 dilution of the original infected mouse brain was infective for mice. 2) The infected culture cells exhibited characteristic degeneration indistinguishable from that associated with infection of tissue cultures with type 1 dengue virus. Infectivity of the virus was neutralized by specific antiserum as indicated by the fact that the degeneration was suppressed completely by homologous immune rabbit serum, but not by heterologous serum. It appeared that the cellular degeneration produced by type 2 virus mixed with anti-type 1 serum was a little milder than that produced by the same virus mixed with the control non-immune serum. Although further studies are necessary to clarify the antigenic relationships of type 1 and type 2 dengue viruses, it is possible that the reduced degeneration was due to a partial neutralization by heterologous antibody.
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