RNase L activity does not contribute to host RNA degradation induced by herpes simplex virus infection
Open Access
- 1 April 2003
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 84 (4) , 925-928
- https://doi.org/10.1099/vir.0.18695-0
Abstract
In early herpes simplex virus (HSV) infection, the virion host shutoff (vhs) protein mediates the degradation of mRNA and subsequent shutoff of host protein synthesis. It is unclear whether vhs acts alone or in concert with virus-induced cellular factors for this activity. This paper examines whether RNase L, a virally induced endoribonuclease, contributes to HSV-induced mRNA decay. Results showed that RNA degradation was comparable in wild-type and RNase L−/− cells, demonstrating that HSV-mediated RNA degradation is independent of RNase L activity. Furthermore, the data show that HSV-1 does not significantly induce RNase L activity in murine embryo fibroblasts.Keywords
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