Metabolic pathways in Ehrlich ascites tumor cells recovering from a low bioenergetic status
- 22 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (2-3) , 183-186
- https://doi.org/10.1016/0014-5793(94)00759-4
Abstract
Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightly inhibited by oligomycin. Cells resuspended in a glucose‐free medium initially showed high dye uptake and ATP level, which were stable over time: in this case oligomycin caused a drop in both dye uptake and ATP level. The above findings, which are indicative of a marked Crabtree effect in Ehrlich ascites tumor cells, means that it is unlikely that limiting ADP and Pi play an important role in the glucose‐induced inhibition of oxidative phosphorylation in this system.Keywords
This publication has 19 references indexed in Scilit:
- Glucose decreases respiratory control ratio in EL‐4 tumor cellsFEBS Letters, 1992
- The impairment of respiration by glycolysis in the Lewis lung carcinomaCancer Letters, 1990
- Ca++-ionophore-induced dissipation of intracellular proton gradients in rat thymocytesJournal of Cellular Physiology, 1989
- P-Nmr-Spectroscopy Measurements of Energy Metabolism of in Vivo Growing Ascites Tumours Following Addition of GlucoseActa Oncologica, 1989
- The role of high rates of glycolysis and glutamine utilization in rapidly dividing cellsBioscience Reports, 1985
- Electrogeneicity of the lysosomal proton pumpFEBS Letters, 1984
- Evidence for an ATP-driven “proton pump” in rat liver lysosomes by basic dyes uptakeBiochemical and Biophysical Research Communications, 1979
- Rapid loss of ATP by tumor cells deprived of glucose: Contrast to normal cellsBiochemical and Biophysical Research Communications, 1978
- The absence of glucose in ehrlich ascites tumor cells and fluidBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- Über den Stoffwechsel von Tumoren im KörperJournal of Molecular Medicine, 1926