Detection of Human Spermatozoal Peptides After Conjugation to 125J-Labelled Human Serum Albumin

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Abstract
Human spermatozoal peptides, liberated during autolysis of the cells, were fractionated by gel-filtration chromatography and TLC. After conjugation to 125I-labeled human serum albumin, all fractions were assayed with rabbit antihuman spermatozoa antiserum. The reactions indicated the presence of a number of antigenic peptides in the spermatozoal autolysate and 1 of the fractions revealed a significantly higher antibody binding capacity than all other fractions assayed (P < 0.05). Release of immunogenic peptides during autolysis of the spermatozoal membrane could be important in the etiology of spermatozoal iso- and autoantibodies.