Growth Inhibition of Tobacco Tissue Cultures with 6-Azauracil, 6-Azauridine and Maleic Hydrazide

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Abstract
The growth effects of 6-azauracil, 6-azauridine and maleic hydrazide were determined with tissue cultures of Nicotiana species and a tumor-forming hybrid. The inhibition of all cell types was complete at 10-4 molar concentrations. The inhibition could be completely relieved with uridine and only partially overcome with uracil. The growth inhibition with maleic hydrazide was irreversible which indicates a mechanism or level of inhibition different from that of 6-azauracil. This study supports the concept that derivatives of 6-azauracil inhibit the synthesis of uridine derivatives required for ribose nucleic acid synthesis and growth. Probable sites of action and feedback phenomena are discussed.