Protein synthesis in ripening pea seeds. 1. Analysis of whole seeds

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Abstract
Extracts of Pisum sativum at 7 different stages of development were analyzed for total N, non-dialyzable N, total and non-dialyzable amino N and total and non-dialyzable amide N; total non-protein N and peptide N were determined by difference. The protein distribution in the extract was determined from ultracentrifuge diagrams. In the early stages of development the incoming N was distributed about equally among the different fractions. With ensuing desiccation, however, there occurred first a sharp increase in peptide N and later a sharp increase in protein N at the expense of peptide N. The data offer strong evidence in favor of peptide intermediates in the synthesis of the seed protein.