Uptake of fibrillar β-amyloid by microglia isolated from MSR-A (type I and type II) knockout mice

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Abstract
To charaterize the receptors involved in binding fibrillar amyloid A-beta (fAβ), we compared the uptake of fAβ in microglia from wildtype (MSR-A+/+) and MSR-A knockout (MSR-A−/−) mice. On average, there was a 60% reduction in the uptake of Cy3-fAβ in microglia from the MSR-A−/− mice. Cy3-fAβ uptake in the MSR-A−/− mice was still competable by scavenger receptor ligands, including acetylated low-density lipoprotein (Ac-LDL) and fucoidan. This indicates that uptake by MSR-B and/or other MSRs is also involved in the uptake of fAβ by microglia. However, the significant reduction in the uptake of fAβ in the MSR-A−/− microglia suggests that fAβ gets internalized mostly by MSR-As in microglia. Uptake of modified fAβ (C1qAβ) was similar in the MSR-A−/− microglia as in the wildtype indicating that the uptake of the opsonized fAβ is independent of MSR-A.