Simplified, Efficient System for In Vivo Mass Production of Gypsy Moth Nucleopolyhedrosis Virus12

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Abstract
Efficient methods for mass production of Lymantria dispar (L.), nucleopolyhedrosis virus (NPV) were developed. Fifth-stage larvae were infected with NPV and held at 29°C until ca. 75% of the insects were dead. Larvae were then frozen (−20°C) for 18 h, lyophilized, and transferred to an apparatus that dislodged the allergenic, urticarious setae. The final step in the production system involved milling the lyophilized larvae to obtain a virus powder. The freeze-drying dehairing-milling technique was less costly than the previous system and produced a technical product with fewer contaminants and virtually no larval setae.

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