Enhanced acetylcholine secretion in neuroblastoma x glioma hybrid NG108‐15 cells transfected with rat choline acetyltransferase cDNA

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Abstract
Neuroblastoma x glioma hybrid NG108‐15 cells and mouse neuroblastoma N18TG‐2 and NIE‐115 celIs were transiently transfected with the sense cDNA coding for rat choline acetyltransferase (ChAT). All transfected cell lines showed a high level of ChAT activity. ACh secretion was monitored by recording miniature end‐plate potentials (MEPPs) in striated muscle cells that had been co‐cultured with transfected cells. The number of muscle cells with synaptic responses and the MEPP frequency were higher in co‐culture with transfected NG108‐15 cells than with control or mock cells. No tsynaptic response was detected in muscle cells co‐cultured with transfected N18TG‐2 or N1E‐115 cells. The results show that ACh secretion into the synaptic cleft was enhanced due to ChAT overexpression in NG108‐15 hybrid cells but not in neuroblastoma cells.