Detection of an Antigen in Albumin Subjected to Repeated Heating

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Abstract
Stabilized 5 and 25% normal serum albumin (human) derived from plasma, placentas and plasma-placental blends was subjected to repeated heating at 56.degree. C for 120 h, interspersed with storage at 4.degree. C for 48 h. Immunoelectrophoretic analyses showed that after the 9th heating, 5% plasma albumin developed a component which migrated in the .alpha.-globulin region and gave a reaction of nonidentity with albumin. This antigen, which was not detected in the other albumin samples, reacted with [rabbit] antiserum prepared against control 5% plasma albumin, indicating that it was present in the latter at an undetectably low concentration.

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