Optimization of a fermentation medium for the production of Penicillin G acylase from Bacillus sp.
- 1 December 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 35 (6) , 523-527
- https://doi.org/10.1046/j.1472-765x.2002.01234.x
Abstract
Optimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp. Fermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components. A maximum production of 9.5 U ml(-1) of PAC was obtained in an optimized medium containing (g l(-1): K2HPO4, 1.0; MgSO4.7H2O, 0.1; CaCl2.2H2O, 0.1; PAA, 2.0; tryptone, 5.0; yeast extract, 3.0; and sucrose, 50.0. The two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.Keywords
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