Isolation and Structure of the Amino-Terminal Cross-linking Region in Insoluble Type III Collagen
- 1 January 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (2) , 1409-1416
- https://doi.org/10.1515/bchm2.1976.357.2.1409
Abstract
A collagenous peptide T1X was isolated from a tryptic digest of the insoluble matrix of calf skin. The peptide consists of 2 identical polypeptide chains each with a length of 72 amino acid residues joined by a cross-link. Absorption spectra obtained from hydrazone and azine derivatives of T1X indicated that the peptide contains an aldol-type of cross-link (X). The sequence of 23 amino acid residues in the amino-terminal region was determined as Glx-Tyr-Glu-Ala-Tyr-Asp-Val-X-Ser-Gly-Val-Ala-Gly-Gly-Gly-Ile-Ala-Gly-Tyr-Hyp-Gly-Pro-Ala. This sequence overlaps the previously described amino-terminal sequence of .alpha.1 (III) chain obtained from pepsin treated, insoluble type III collagen. The present data demonstrate a nonhelical segment of 14 amino acid residues in type III collagen important for cross-linking.This publication has 14 references indexed in Scilit:
- Aldehyde content and cross-linking of type III collagenBiochemical and Biophysical Research Communications, 1976
- [α1(III)]3 Human Skin CollagenPublished by Elsevier ,1974
- Human aorta collagens: Evidence for three distinct speciesBiochemical and Biophysical Research Communications, 1974
- Staphylococcal Protease: A Proteolytic Enzyme Specific for Glutamoyl BondsProceedings of the National Academy of Sciences, 1972
- Structure and Metabolism of Connective Tissue ProteinsAnnual Review of Biochemistry, 1972
- Relationship between the Intra and Inter molecular Cross-links of CollagenProceedings of the National Academy of Sciences, 1970
- A reinvestigation of the hydrazinolytic procedure for the determination of C-terminal amino acidsArchives of Biochemistry and Biophysics, 1967
- The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgGBiochemical Journal, 1966
- Determination of carbonyl compounds with N-methyl benzothiazolone hydrazoneArchives of Biochemistry and Biophysics, 1965