DENGUE VIRUS-INDUCED THYMUS-DERIVED SUPPRESSOR CELLS IN THE SPLEEN OF MICE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (4) , 653-658
Abstract
Adoptive transfer of spleen cells obtained from mice given 3 weekly i.p. doses of dengue type 2 virus (DV) suppressed DV antigen-specific antibody secretion as detected by the Jerne plaque technique. This suppression was produced by non-glass-adherent cells but not by glass-adherent cells. Immune spleen cells depleted of macrophages by carbonyl Fe treatment had higher suppressor activity. Immune spleen cell homogenate could transfer the activity equally well. The immune spleen cells were separated into T [thymus-derived] and B [bone marrow-derived] lymphocytes by a nylon wool column. B lymphocytes had no suppressor activity; almost all the suppressor activity was present in T lymphocytes. Macrophages and B lymphocytes had no suppressor activity; it was mediated by T lymphocytes through soluble factors.